- #Serial dilution lab report microbiology serial
- #Serial dilution lab report microbiology series
The same process is then repeated for the remaining tube, taking 1 ml from the previous tube and adding it to the next 9 ml diluents. The second tube now has a total dilution factor of 10 -2. Now, 1 ml of mixture is taken from the 10 -1 dilution and is emptied into the second tube. View Lab Report - micro lab report -serial dilution.docx from MBIO 4823 at The University of Oklahoma. The pipette tip is discarded, and a new pipette tip is attached to the pipette. The dilution is thoroughly mixed by emptying and filling the pipette several times. This provides an initial dilution of 10 -1. The sample is then added to the first tube to make the total volume of 10 ml. 1 ml of properly mixed sample/culture is drawn into the pipette. The sample/culture is taken in a test tube and six test tubes, each with 9 ml of sterile diluents, which can either be distilled water or 0.9% saline, are taken. The following is the procedure for a ten-fold dilution of a sample to a dilution factor of 10 -6: Total dilution factor = previous dilution × dilution of next tube Total dilution factor for the second tube = dilution of first tube × dilution of the second tube.įor the first tube, dilution factor = 10 -1 (1 ml added to 9 ml)įor the second tube, dilution factor = 10 -1 (1ml added to 9 ml). After the first tube, each tube is the dilution of the previous dilution tube. 
In this case, the dilution factor for that test tube will be: Next, we will vortex this solution for 3o seconds and removed it and then vortex it for another 30 seconds.
#Serial dilution lab report microbiology serial
The dilution factor of each tube in a set:įor a ten-fold dilution, 1 ml of sample is added to 9 ml of diluent. Methods and procedures Serial dilutions: Starting with 1ml of red food dye, we will add it to test tube1 along with 9 ml of distilled water.The dilution factor in a serial dilution can be determined either for an individual test tube or can be calculated as a total dilution factor in the entire series.Serial two-fold and ten-fold dilutions are commonly used to titer antibodies or prepare diluted analytes in the laboratory.In contrast, for a less contaminated sample, a low dilution factor might be sufficient. For e.g., if a water sample is taken from an extremely polluted environment, the dilution factor is increased. Depending on the estimated concentration of cells/organisms in a sample, the extent of dilution is determined.As it would usually be impossible to actually count the number of microorganisms.
#Serial dilution lab report microbiology series
Then, a small measured volume of each dilution is used to make a series of pour or spread plates. Serial dilutions are used to calculate the concentration of microorganisms. Serial dilution involves the process of taking a sample and diluting it through a series of standard volumes of sterile diluent, which can either be distilled water or 0.9 % saline.
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